CHAPTER 14;  ALDF/IDSA Refer to the URI's Cyst-Reverts-to-Intact-Spirochete-Form report, but have never used the Mouse Infectivity Test (transfer of spinal fluid containing cysts, since spinal fluid is a "serum-starvation" condition) to assess antibiotic treatment outcomes.

The crooks say the cyst is not viable, without performing a MIT.

The CDC says spirochetes are intracellular in 2006:
http://www.ncbi.nlm.nih.gov/pubmed/17045505?ordinalpos=13&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum
Invasion of human neuronal and glial cells by an infectious strain of Borrelia burgdorferi.

 

URI's Dave Nelson "Reversion of cyst form to intact spirochetes within one minute of addition of rabbit blood:"

 http://mic.sgmjournals.org/cgi/reprint/146/1/119  (This was published in the United States in recent time so the CDC can't reject it which is what they always do with data they don't like from Europe.)

 

What to know:  The Mouse Infectivity Test and the cyst or spheroplast- You will like this very revealing report

The transfer of spinal fluid from one mouse to another is the best way to infect another mouse and prove infection in the first mouse (treatment failure).  What's in the spinal fluid is cysts, since the spirochetes revert to cyst form in spinal fluid, as that would be a "serum-starved" environment.

http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9774573

Detection of active infection in nonhuman primates with Lyme neuroborreliosis: comparison of PCR, culture, and a bioassay.

(Philipp, Mario, Tulane)

J Clin Microbiol. 1998 Nov;36(11):3243-7.

"Ideally a diagnosis of infection of the central nervous system (CNS) is made by culture of the etiologic pathogen, but Borrelia burgdorferi, the causative agent of Lyme neuroborreliosis (LNB), is rarely cultured from the cerebrospinal fluid (CSF). PCR and measurement of specific antibody in the CSF also have their limitations. The role of available assays for LNB has not been studied carefully in a comparative investigation. There is a need to assess the reliability of assays and to increase the ability to document active infection in the CNS. The recent development of the nonhuman primate (NHP) model of LNB allowed us to address this need in a faithful model of human LNB. In this study we compared the abilities of PCR and culture to detect the presence of spirochetes in the CSF and brain tissue of infected NHPs and related these measures of infection to the development of anti-B. burgdorferi antibody. We also tested a bioassay, the mouse infectivity test (MIT), in this model. Fourteen of 16 CSFs from four NHPs were positive by at least one of these techniques. Detection of spirochetes in the CSF by PCR, the MIT, and culture was inversely related to the concomitant presence of anti-B. burgdorferi antibody intrathecally. The performance of any particular test was associated with the strength of the host immune response. In early CNS infection, when anti-B. burgdorferi antibody had not yet appeared, or in immunocompromised hosts, the MIT compared favorably to culture and PCR for infected NHPs; antibody in the CSF was the most useful assay for immunocompetent NHPs."

 

We learned in Chapters 3 and 10 that people with chronic Lyme are immune-suppressed.

 

FROM the 1989 IDSA "REVIEWS":
RUSSELL JOHNSON, CULTURING - spheroplasts could take months (and not weeks) to revert to intact spirochetes in BSK media:

 

The following is the data which shows www.IDSociety.org knows the spheroplast or the cyst form of the spirochete is viable.   The Mouse Infectivity Test is the only test to use when assessing treatment outcomes.

First, understand that mice are not the best model for human disease:

'Storing Spirochetes in rodent brains, Oscar Felsenfeld: "mouse model of rodent brain infection is no good."

That's why the crooks always use mouse model ;-)

http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=441260&blobtype=pdf

 

 

Exposure to antibiotics causes the spirochete to go into the spheroplast stage, which the bad guys say is the end stage, yet they know it isn't. 

Here is CDC officer Alan Barbour demonstrating that he knows antibiotics cause the spheroplast form to occur upon exposure to antibiotics:

http://aac.asm.org/cgi/reprint/21/5/823?view=long&pmid=7103461
(Hermsii is the closest relative to burgdorferi when assessing flagellin DNA differences, Picken, 1992.)

◄ "adverse conditions"-  this is what it is- an "adverse conditions" form and not necessarily a life cycle stage.

Additionally, all the crooks referenced Dave Nelson's "reversion to intact spirochetes within one minute of the addition of rabbit blood" report, which is also here on the Russian Bioweaponeers at New York Medical College page, as are more of the same reports about the viability of the cyst (or the weaponizable aspect of this bioweapon).

--1911, Andrew Balfour, MD, Khartoum, "Infective granules"

 

--1983, Proposed Life Cycle for the Reiter Treponeme  (validity of "cysts", or spheroplasts. or regeneration forms)

 

--1992, Formation of Multiple Treponemes

RICOCHRON

CHAPTER 15, PLUM ISLAND SLYME (OspA) AND IMMUNE-SUPPRESSION